Chiefs physiotherapist David Milner confirmed the news on the club’s website.
“Itumeleng Khune re-injured his knee during training last week,” said Milner. The surgery will correct a torn meniscus.
Khune had to withdraw from the Bafana Bafana squad to face Angola in a 2018 World Cup qualification match in Benguela on Friday, with the return leg on Tuesday in Durban.
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