South Africa are set to meet Sao Tome at Moses Mabhida Stadium on 13 November and for the return game away three days later on 16 November.
The first leg will be played at 7pm while the match in Sao Tome will kick off at 3pm local time (5pm SA time).
Ntseki has recalled Itumeleng Khune, Bongani Zungu and Thulani Serero who missed this month’s friendlies against Namibia and Zambia.
Mamelodi Sundowns utility player Lyle Lakay has also made it to the squad, with Ntseki saying that he included him because he wanted more left-footed players in the team to find the right balance.
The Bafana squad:
Goalkeepers: Darren Keet, Ronwen Williams, Itumeleng Khune
Defenders: Thibang Phethe, Motjeka Madisha, Thulani Hlatshwayo, Lyle Lakay, Innocent Maela, Rushine De Reuck, Abbubaker Mobara, Reeve Frosler
Midfielders: Andile Jali, Themba Zwane, Thulani Serero, Teboho Mokoena, Bongani Zungu, Keagan Dolly, Percy Tau, Lebogang Manyama, Kamohelo Mokotjo, Luther Singh, Dean Furman
Strikers: Kermit Erasmus, Bradley Grobler, Thabiso Kutumela