Matsatsantsa will miss the influential Aubrey Modiba when they face neighbours Mamelodi Sundowns at Lucas Moripe Stadium on Saturday night.
Earlier that day, Bakgaga will be without Goodman Mosele, while Rise and Shine will have to do without the services of Sibusiso Mbonani when the sides meet at Peter Mokaba Stadium.
Also on Saturday, Cape Town City and Bidvest Wits will miss Roland Putsche and James Keene respectively when they clash at the Athlone Stadium.
AmaZulu will travel to Bethlehem to face Free State Stars without Michael Morton and Butholezwe Ncube. Both players are suspended for this clash.
On Sunday, Chippa United will have to do without the services of their defender Zitha Macheke when they host Orlando Pirates at the Nelson Mandela Bay Stadium.
At the same time, Golden Arrows will miss Jabulani Shongwe when they play Ajax Cape Town at the Princess Magogo Stadium.
Tonight, Maritzburg United will face struggling Platinum Stars at Harry Gwala Stadium without Lebohang Maboe.