Matfield, last captained the Bulls in the opening round of the competition before knee surgery sidelined the Springbok lock for a month. Grant Hattingh as a result drops to the bench.
In total the Bulls made five changes to their side which lost 15-13 to the Stormers at Newlands, in Cape Town, last week.
Back in the starting XV are Jan Serfontein, Arno Botha, Lappies Labuschagne and Trevor Nyakane.
Serfontein was a late withdrawal from the team that played the Stormers last week, while Botha, Labuschagne and Nyakane move up from the bench to take their starting positions.
Burger Odendaal drops to the bench for Serfontein and Botha takes up the number eight position for Pierre Spies takes a seat among the replacements.
Nyakane comes in for Dean Greyling who suffered a minor knee injury in a car accident on Wednesday.
Labuschagne replaces Deon Stegmann who was rested after 10 consecutive starts for the Bulls.
Prop Andrew Beerwinkel was also named in the replacements.
Bulls team: 15 Jesse Kriel, 14 Francois Hougaard, 13 JJ Engelbrecht, 12 Jan Serfontein, 11 Bjorn Basson, 10 Handré Pollard, 9 Piet van Zyl, 8 Arno Botha, 7 Jacques du Plessis, 6 Pieter Labuschagne, 5 Victor Matfield (captain), 4 Flip van der Merwe, 3 Marcel van der Merwe, 2 Adriaan Strauss, 1 Trevor Nyakane. Replacements: 16 Callie Visagie, 17 Morné Mellett, 18 Andrew Beerwinkel, 19 Grant Hattingh, 20 Pierre Spies, 21 Tian Schoeman, 22 Burger Odendaal, 23 Jurgen Visser.