Joining Lusaseni in a strong side with several Super Rugby experience are wing Anthony Volmink, centre Alwyn Hollenbach and hooker Malcolm Marx.
The selection of blindside flanker Ruaan Ackermann, son of Lions Super Rugby coach Johan Ackermann, will also cause interest, while others like blindside flanker Steph de Wit and No 8 Ruaan Lerm were part of the Super Rugby training group.
The tall 2,11m lock JP du Preez, who made his Super Rugby debut for the Lions two weeks ago against the Sharks in Durban finds himself on the bench.
SA Sevens player Mark Richards, who covered several games for the Lions last season at scrumhalf, has been named on the wing.
Golden Lions head coach Russell Winter announced his team to face Welwitschias in the first Vodacom Cup match of the season on Wednesday afternoon.
The match will be played in Windhoek, Namibia on Friday at 7pm.
The team will be captained by scrumhalf Ricky Schroeder who also led them with distinction last season, while also featuring on occasion for the Lions in Super Rugby.
Golden Lions: Damian Engledoe, Mark Richards, Jacques Nel, Alwyn Hollenbach, Anthony Volmink, Ashlon Davids, Ricky Schroeder (capt), Ruaan Lerm, Ruan Ackermann, Steph de Wit , MB Lusaseni , Bobby de Wee , Pieter Scholtz, Malcolm Marx, Dylan Smith.
Bench: Ramone Samuels, Clinton Theron, JP du Preez, Cyle Brink, Lohan Jacobs, Rohan Janse van Rensburg, Vainon Willis