SABC radio reported that police dog unit investigator Sergeant Jerome Timmy testified that he believed there was not enough blood spatter at the scene to indicate the attack took place there.
Johannes Kana is on trial for allegedly raping and disembowelling Booysen, 17, in Bredasdorp, in the Western Cape, in February. Booysen died in Tygerberg Hospital on the afternoon of Saturday, February 2.
He has admitted leaving a Bredasdorp pub with Booysen, hitting and raping her, but has denied killing her.
According to the broadcaster, the court heard that a baseball cap, allegedly belonging to Kana, had tested positive for traces of blood.
The investigating officer in the case was expected to testify when the case resumed on Wednesday.