She is accused of submitting forged documents to obtain Construction Industry Development Board gradings, which were then used to win five public works department tenders worth R140 million.
Mpisane, who is out on R100,000 bail, faces 53 charges of fraud, forgery, and uttering of a forged document.
The Mercury newspaper reported on Tuesday that Mpisane and her husband S’bu had failed in their attempt to overturn a R70 million restraint order on their assets.
The order was granted last year and was initially for R140m, but was later halved.
According to the report, Judge Sharmaine Bolton rejected Mpisane’s argument that material information not provided to the court would have affected its original decision to grant the restraint order.
She also rejected their argument that it was necessary there be reasonable prospects for conviction for such an order to be granted.