Tshwane Emergency Management Services (EMS) spokesperson Johan Pieterse said one bus had collided into the back of another which had broken down, according to eyewitnesses.
Pieterse confirmed that the driver of the bus that rear-ended the stationary vehicle had been killed in the crash.
“Eight people sustained serious injuries, while 33 were slightly injured,” he said.
Moloto Road, dubbed “the unholy road”, has a long history of fatal crashes.
The deadly crashes had persisted on the 130km-long road linking Pretoria and Mpumalanga despite several interventions by the Department of Transport.
Earlier this year, four people were killed and 12 others injured when a taxi overturned on the R573 Moloto Road near Mathyzenloop, Mpumalanga.
Three passengers and the driver died at the scene.
In November, A bus collided with a truck killing 29 people and severely injuring a further 11.
The accident occurred when an articulated vehicle drove into the back of a tipper truck before colliding with a bus and a light delivery vehicle.
Transport Minister Dipuo Peters had ordered a high-level investigation into the accident by the Road Traffic Management Corporation and Mpumalanga traffic authorities.
Earlier this month, DA Gauteng Shadow roads and transport MEC, Neil Campbell, said what distinguished this road from others, was that the road itself was not risky, but rather because of the “appalling attitude of those who use the road and their flagrant disregard for any principle of road-safety”.
“What is needed are dedicated police officials who apply appropriate policing policy for road violations and ensure that perpetrators are brought to book,” said Campbell.
Twitter users react to the latest crash:
— RoadSmart SA (@RoadSmartSA) September 30, 2014
— Keitu Kovac (@Kei2M) September 30, 2014
Unholy road that. "@Tebogo_Bone: Moloto Rd tho, always has these accidents with high death tolls… And 1 in 4 involve a Putco bus"
— Lola (@LolaMonareng) September 30, 2014
Terrible accidend ko moloto road @ScHooLBoY_SpM: Ehhh why? @kabelomk5"
— Kaybee (@kabelomk5) September 30, 2014
Moloto road should be turned into a freeway once n for all.
— Ningi Nice ???????????? (@Ningi_Nice) September 30, 2014
Moloto road RT @SA_Trivia: 4. Whats the major road in Mpumalanga with the most car accidents
— Khomotjo???? (@KhomotjoM) September 23, 2014