At least 30 boys died at seven initiation schools in Kwaggafontein, KwaMhlanga, Verena, Delmas, Siyabuswa, Belfast and Middelburg last year.
“The police acted swiftly by opening dockets and commenced with the investigations,” National Prosecuting Authority spokesman Nathi Mncube said.
The 23 would face a range of charges.
“In separate trials, four medical doctors, 18 traditional circumcisers and one circumcision principal stand to be tried on 24 counts of culpable homicide, two counts of assault with intent to do grievous bodily harm and 12 counts of contravening… the Children’s Act.”
The act prohibits the circumcision of children below 16 and allows for the cirumcision of boys between 16 and 18 only in certain circumstances.
Inquests would be held in three of the deaths.