Supa Zuma, the party’s secretary for the Moses Mabhida region, said in a statement that Nkosi Nkwanyana was gunned down outside his home in the Mhlangandlovu area.
Jabulile Ncalane, a party volunteer, was hacked to death in her house a week earlier.
The Mhlangandlovu area is in the uMshwathi local municipality in the KwaZulu-Natal midlands.
Zuma said the ANC condemned the attacks and that to date no arrests had been made.
Comment could not immediately be obtained from police.