Two children, aged five and 10, had been quarantined with cholera at Beitbridge District Hospital, the official Herald reported.
Six other cases had been recorded in Mudzi and Chiredzi, the state Zimbabwe Broadcasting Corporation said.
Mudzi is on the border with Mozambique, where 41 people have died of cholera. Chiredzi is in south-eastern Zimbabwe.
Another possible case was being investigated in Chirundu on the Zambian border, the report said.
Beitbridge district administrator Simon Muleya told the Herald: “The two victims are from the same family and according to our rapid response team there are no other cases from the same village.
“The situation is now under control and there is no need for people to panic,” he said.
The children were reportedly in a stable condition in the Cholera Isolation Centre of Beitbridge District Hospital.
Cholera killed more than 4000 people in Zimbabwe in late 2008 to early 2009. The disease spreads particularly fast during the rainy season through contaminated food and water.